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Following ligation, the researcher transformed a competent strain of with the ligation reaction and plated the cells onto culture media containing X-gal and ampicillin. After plating, the researcher realised that IPTG had been omitted from the culture media and all the colonies obtained were white.
Q29. Based on these results, can you conclude whether the E. coli cells were successfully transformed with the recombinant plasmid? Explain why or why not. (1-2 sentences) (2 marks)
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