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Denaturation  >  Annealing  >  Elongation

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To determine if a DNA sequence contains a single nucleotide polymorphism

To separate DNA fragments on the basis of their charge

To determine the sequence of nucleotides in a DNA fragment

To amplify several DNA fragments at once

To separate DNA fragments on the basis of their size

To remove any mutations in the genes

Before amplification, DNA fragments are likely to bind to RNA and would no longer be able to be analysed

A gene may represent only a millionth of the cell's DNA

To remove the negative charge on the DNA, to enable them to be analysed

To make seeing the nucleotide sequence easier