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Why is a reference genome (e.g., GRCh38) used in RNA-seq analysis?

A. To

reconstruct expressed proteins

B. To

identify the genomic position of sequencing reads

C. To

measure cell size

D. To

detect only mitochondrial genes

E. To

remove PCR duplicates

Why is single-cell RNA-seq

particularly suitable for studying

cellular heterogeneity?

C. It

removes non-viable cells automatically

D. It

amplifies genes expressed only in tumor cells

A. It

sequences the proteins produced by each cell

B. It

allows observation of gene expression differences between individual cells

E. It

measures the total amount of RNA in a tissue

Which indicator is commonly used to assess

reproducibility between

RNA-seq replicates

In transcriptomic analysis, deconvolution allows researchers to:

Estimate the contribution of different cell types in a mixed sample

Identify subcellular RNA localization

Reconstruct mitochondrial genomes

Identify cancer mutations

Measure transcription speed

Which region corresponds to the

transition zone between the tumor and

healthy tissue

How do T cells recognize tumor cells?

DNA–histone interaction

Cytokine–nuclear receptor interaction

RNA–ribosome interaction

B. TCR

interaction with antigen presented by MHC I

Antibody–antigen interaction

In 10x Genomics

platforms, why is a large number of barcodes

required?

E. To

reduce read length

D. To

improve the quality of the reference genome

B. To

distinguish a large number of individual cells

C. To

identify genetic mutations

A. To

increase sequencing depth

Which step is essential before library preparation in single-cell RNA-seq?

What is the role of cellular barcodes

in single-cell RNA-seq

experiments?

C. To

amplify low-expressed genes

B. To

identify the cell of origin of each RNA molecule

A. To

identify genetic mutations

D. To

remove bacterial contamination

E. To determine transcript length