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BIO1011 - Blueprints for Life - Clayton S1 2025
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The cell/plasma membrane of a bacterium living in a salty aquatic environment contains a membrane channel protein called MelB. When this bacterium is placed in a hypertonic solution that models/mimics its natural habitat, MelB facilitates the uptake of the sugar melibiose along with sodium ions (Na+).a) What potential challenges might this organism encounter if it were deficient in membrane proteins responsible for moving charged particles across this boundary? Consider the salty environment (75 words, 1.5 marks).b) Explain how MelB functions to transport melibiose and sodium ions (Na+) across the membrane and what type of cellular transport mechanism does it use? (25 words, 1 mark).
The hypothetical scenario has the major drawback in that it will only provide qualitative data, as opposed to the quantitative data, that the original method allows.Outline the difference between qualitative data and quantitative data, using a specific example from the Microbial Water Testing lab class (100 words).
In reference to the hypothetical scenario above, outline how you would prepare an appropriate positive and negative control (100 words).NB: Your answer MUST include a description of the expected result for BOTH the positive and negative controls AND how each would be prepared (i.e. what would the control actually contain). It is not required to specifically name any bacterial species and enzymatic activity can simply be stated as beta-galactosidase positive or beta-galactosidase negative.