A researcher studying enzyme kinetics created the following series of Michaelis-Menten ("MM") and Lineweaver-Burk ("LB") plots, shown below, but forgot which MM plot went with which LB plot.

Can you help them out and correctly pair up the graphs?

In the process of revising for BCH2011, you fell asleep (understandable) but upon awakening you realised you dreamt of the titration of an amino acid side chain (understandable). The details of the dream are murky, but you recall it looking something like this:

What is the pKa of the side chain and which amino acid were you dreaming of?

The data below shows four different protein unfolding curves for the same protein but containing various mutations.

The protein is 280 amino acids long. The N-terminal region of the protein involves a cluster of hydrophobic amino acids critical for stabilising the overall structure of the protein. The C-terminal region contains a solvent-exposed pocket containing charged amino acids that, whilst not so critical for protein folding, play an important role in the protein's function.

The mutations studied were as follows:

• R247K
• F38V
• F38A

The wild-type (WT) version of the protein has been extensively studied and no mutation has ever been found that has improved stability.

Which condition corresponds to which version of the protein?

Oh look, it's the same sequence again.

Suppose that an A was introduced after codon 2 but before codon 3.

Suppose also that the final nucleotide of codon 4 was deleted.

Which statement below is true?

Let's start easy with something easy.

Here's a coding strand sequence, with each codon helpfully spaced by spaces and each codon numbered by numbers and with me writing more words than are necessary as some introductory text.

Spot the stop.

Good, very good.

Let's remain with that same sequence, but crank things up a notch.

Change to a different branched amino acid. (We know which those are, right? ... Right?)

OK, so here's that same image again.

What amino acid is circled? Enter your answer as the full amino acid name. E.g. if you think it's F, then write Phenylalanine.

Note also that nitrogen is blue, oxygen is red. Carbons (in this bit) are green. No hydrogens are shown.

Here we are again, staring at an amino acid, its backbone emerging from the cartoon of the beta strand like a whale breaching the pristine seas.

What R group do you see here?

Here's an image from that same protein, zooming in on a beta turn and with four amino acids highlighted in ball and stick representation. I've drawn around one amino acid.

With respect to the beta turn, which statement describes the circled amino acid?

Let's make it a little trickier with this image:

Which option is the correct sequence of the three amino acids shown here, in N- to C- direction?

Take your time. Think about where you can look to work out the correct direction through the alpha helix. There's one specific location that gives you everything you need.