Shown below are three short DNA duplexes.

What is the correct order of stability of these duplexes (from most stable to least stable)

[Note: You do not need to calculate T_m values. You should be able to determine the order of stability “by eye”.]

How many micromoles (μmol) of ATP are present in 3 mL of 25 mM ATP solution?

An experiment requires you to create a stock buffer containing 0.04 M of NaOH in a total volume of 450 mL.

How many grams (g) of solid NaOH are required to prepare this stock solution?

Note: Your answer should be to two decimal places, e.g. 4.31

5 mL of a stock solution of 600 mM citrate buffer is diluted to a final volume of 100 mL.

What is the final concentration of the diluted citrate buffer (in units of mM)?

A sample contains 11 μg of NaOH.

How many milligrams (mg) is this?

An experiment requires the use of 20 mmol of sodium EDTA, which has a molecular mass of 372 Da.

What mass of sodium EDTA (in grams, to two decimal places) is needed for this experiment?

A patient has been admitted to hospital and a blood test has shown his glucose level is 40 mg per 45 mL.

Calculate the molarity (in mM, to two decimal places) of the glucose in the patient's blood.

Which of the following statements correctly identifies a hydrogen bond donor and a hydrogen bond acceptor in the base pair structure shown below?

The following sequence of RNA forms a “hairpin” structure. The duplex region contains all Watson-Crick base pairs without any gaps or mismatches.

5′-UGUACGCUAGUGUUAUAUCGCACUAGCUACG-3′

In the space below, type the sequence of either of the two regions that form a duplex with each other.

Be careful not to type any spaces or additional characters. For example, if you think the answer is 5'-AGCC-3', simply type AGCC.