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DEV3011 - Experimental developmental biology and disease modelling - S1 2026

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The image below shows cDNAs on an agarose gel.

image1

 The gel shows the results of what type of experiment?
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The following image represents chromosome pairs from an ENU screen for dominant mutations

in mouse. The mutation was generated on a genetic background represented by the black regions of DNA. This was crossed to a wildtype strain represented by white regions of DNA.

 Define the region of the chromosome pair (A, B, C or D) that contains the mutation.

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Two morphogens are produced along the anterior-posterior axis of the Drosophila embryo.

Morphogen 1 is expressed most highly at the anterior pole, tapering with distance from its source.

Morphogen 2 is expressed highly at the posterior pole, also tapering with distance from its source.

Expression of Gene 3 is stimulated only by intermediate levels of morphogen 1.

Expression of Gene 4 is inhibited by any expression of gene 3 and morphogen 2.

Expression of Gene 5 is stimulated only by intermediate and high levels of morphogen 1.

Expression of morphogen 2 is inhibited by any level of gene 5 expression.

In the figure above, the arrow denotes the expression of which gene or morphogen?

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You are a researcher investigating signalling pathways in neurons. You have detected the presence of Gli repressor protein in the nucleus of the neuronal cells you are studying.

What does this indicate?
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You find that a gene called Scute is expressed in the forelimb bud. Mis-expression of Scute in the flank of a chicken embryo results in an extra (ectopic) forelimb bud. In a separate study, you find that conditional deletion of Scute in the forelimb bud does not affect limb development. What do these findings demonstrate?
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The following image represents chromosome pairs from an ENU screen for dominant mutations

in mouse. The mutation was generated on a genetic background represented by the black regions of DNA. This was crossed to a wildtype strain represented by white regions of DNA.

 Define the region of the chromosome pair (A, B, C or D) that contains the mutation.

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Which description below characterises ENU mutagenesis in the mouse?
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A cell carries a mutation that renders MAP kinases inactive. What would be the result?
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The image below shows cDNAs on an agarose gel.

image1

 The gel shows the results of what type of experiment?
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A Drosophila larva has a loss-of-function mutation in the segment polarity gene, engrailed, that prevents the encoded protein binding to its targets. What the most likely outcome?
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