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DEV3011 - Experimental developmental biology and disease modelling - S1 2026

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Imagine you are studying neural tissue specification in the Xenopus model. The dorsal (“top”) part of the Xenopus blastula, called the “animal cap”, normally forms ectoderm-derived neural tissue and epidermis.

When the animal cap is cut away from the rest of the blastula and grown by itself in culture, it only forms epidermis. This suggests some sort of secreted factor is required for induction of neural tissue. You add a growth factor protein called Noggin to the cultured animal cap and carry out staining for a marker of neural tissue. This is the result, where positive staining is dark:

What conclusion can be drawn? Choose the CORRECT answer.

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Consider the figure below.

What type of molecule is denoted by the black arrow? Choose the CORRECT answer

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The image below shows a gastrulating embryo of the invertebrate, Amphioxus.

What is the name given to the layer of cells denoted by the arrow? Choose the CORRECT answer.

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The image below shows an early chicken embryo during cleavage.

Which type of cleavage is depicted? Choose the CORRECT answer

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Which is the ligand in the image below (A, B ,C or D)?

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GSK3-inhibiting drugs have been extensively studied as therapeutics for the treatment of Alzheimer’s’ Disease, diabetes, and some forms of cancer. In the context of intracellular signalling, what effect would a GSK3 inhibitor have in the embryo?
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Fibroblast Growth Factors are proteins.
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Craniosynostosis describes premature fusion of cranial bones in embryonic life. Most syndromic cases (those where other effects are also seen elsewhere in the body) cannot be explained. However, many can largely be attributed to mutations in genes encoding which group of proteins?
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A high concentration of Sonic hedgehog in the limb bud of a human embryo is responsible for development of the little finger (“pinkie”), but at a lower concentration it does not do so. This is an example of the effects of what type of developmental factor?
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Inhibition of Patched results in inhibition of Hedgehog signalling.
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