β-mercaptoethanol is a component of Laemmli buffer. What is the function of β-mercaptoethanol?

The MFN2 protein is

comprised of six functional domains. Which domain is responsible for anchoring MFN2

in the outer mitochondrial membrane?

What is the

approximate molecular weight of the full-length MFN2 protein in humans?

To study

the processing of

To determine which aspect of carbon catabolism was impacted by

mutagenesis in each of these strains, the strains were also assayed for carbon

catabolism of glucose, xylose and arabinose and sent for genetic sequencing. 

The randomly generated mutants were then

screened for ethanol production. Strains were cultured in media containing

glucose, xylose and arabinose to reflect the composition of agricultural

biomass. The bacterial strains were cultured for a period of fifteen hours and

samples of media were collected at regular timepoints (0, 3, 6, 9, 12 and 15

hours) to track the production of ethanol.

A graph of ethanol production in the PET #A

strain and 2 mutant strains; PET #X, and PET #Y are shown below.

Which strain produced the most ethanol at 15

hours?

The researcher treated PET #A E. coli

with the base analogue

2-Aminopurine which is structurally similar to a nitrogenous base found in DNA.

The structure of 2-Aminopurine is shown below:

The ecologists

used a quantitative PCR (qPCR) assay to investigate the abundance of the novel

organism in several different ecological niches including hydrothermal vents,

deep-sea sediments and shallow coastal waters. The qPCR assay was performed

using a primer that specifically targeted a gene

carried by the novel organism.

Examine the

qPCR results shown above. Which ecological niche (hydrothermal vents, deep-sea

sediments, shallow coastal waters) had the highest abundance of the novel

organism? Briefly explain how you reached this conclusion. (2-3 sentences) (2

marks)

The reagent Vemin8 specifically binds to 7-methylguanosine. 

i) Will Vemin8 bind to myocyte RNA, C. perfringens RNA, or both? Briefly justify your response. (1-2 sentences) (2 marks)

ii) Will Vemin8 bind to small nuclear RNA (snRNA)? Briefly justify your response. (1-2 sentences) (2 marks)

The researcher discovers that expression of the MoxinX  gene is upregulated in infected myocytes. The first 10 nucleotides of the first exon of the MoxinX gene are 5’ ATG GGT TCC T 3’. What is the sequence of the first 10 nucleotides of transcribed mRNA in the pre-mRNA transcript?