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320.004, VU Genomische Datenanalyse, Irene Tiemann-Boege, 2026S
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What is the extinction coefficient of the human PRDM9 in units of M-1 cm-1, assuming all pairs of Cys residues form cystines?
What is the molecular weight of human PRDM9 in units of kDa (based on your results from ProtParam)?
Which protein sequences of PRDM9 are phylogenetically more similar in the alignment?
What is the identity (%) of PRDM9 between Homo sapiens and Rattus norvegicus?
In which tissue is the expression of human PRDM9 RNA enhanced?
According to UniProt, where in the cell is the human PRDM9 located?
What are examples of molecular functions (GO) that the human PRDM9 protein has?
The Uniprot ID for human PRDM9 is
Look at the following multiple sequence alignment from Culstal Omega:
Which pair of primers would you choose to amplify a region of ALL 3 species (the forward primers are highlighted in green, the reverse primers are highlighted in red)?
To get information on the specificity of designed primers, you can use which one of the following specialized BLAST searches: