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BABS1202-Applied Biomolecular Sciences T2 2025

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A bacterial population is monitored for a total time of 20 hours, and it undergoes 5 generations during this period. What is the generation time?

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You have a microbiological spread plate with a dilution factor of 10^4 and a colony count of 170 CFUs. If the plate was inoculated with 100 μL of sample, what was the original viable cell count?

Remember: CFU/ mL = number of colonies x dilution factor / volume plated in mL 

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Five BABS1202 students make an error when performing a Gram stain on a sample containing Gram-positive cocci and Gram-negative rods. Each student makes a different error.

When they looked at their slides under the microscope, only one student did not need to repeat the experiment. Which student will this be?

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You received some agar plates from a manufacturer, but the seal to the packaging is clearly broken, with some of the agar even touching the sides of the packaging.

You inspect the agar plates and there are no visible contaminants, so you decide you need to re-sterlise the plates before use.

What is the method of sterilisation would be best employed to sterilise agar plates already filled with media?

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The method of sterilisation utilising ethanol to treat a surface, sterilises materials through:

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In the process of biofuel (ethanol) production, which of the following occurs after the addition of high starch material?

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Which of the following reagents is NOT included in a PCR master mix?

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What is the electron transport chain?

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You are performing a series of spread plates that require a small volume of diluted bacterial culture. You need 100 µL of the diluted culture for each assay. How many milliliters (mL) of the diluted culture would you need to spread 10 plates?

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Mannitol salt agar contains high salt concentrations that select for Gram positive microorganisms. It also contains mannitol, a carbohydrate that can utilised by some staphylococci to produce an acidic by product, and a pH indicator. This agar can best be described as:

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