Before you can add your recombinantly expressed GFP to the HIC column for purification, you needed to lyse the cells. Which one of the following statements best explains why you did this step?

Question

Before you can add your recombinantly expressed GFP to the HIC column for purification, you needed to lyse the cells.  Which one of the following statements best explains why you did this step?

Answer

To make the proteins more hydrophobic.

Answer

To release the GFP that is expressed within the bacteria.

Answer

To kill the bacteria to prevent contamination of the column.

Answer

To remove all of the bacterial proteins.

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Before you can add your recombinantly expressed GFP to the HIC column for purifi...

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