To test the importance of certain amino acid residues in stabilising a protein structure, a research student performs equilibrium unfolding experiments with three forms of the protein:
(A) the wild type protein;
(B) a mutant in which a stabilising interaction is removed; and
(C) a mutant in which a new stabilising interaction is introduced.
They obtain the three unfolding curves shown below.

Which curves correspond to which forms of the protein?

Question

To test the importance of certain amino acid residues in stabilising a protein structure, a research student performs equilibrium unfolding experiments with three forms of the protein: 
 (A) the wild type protein; 
 (B) a mutant in which a stabilising interaction is removed;  and 
 (C) a mutant in which a new stabilising interaction is introduced. 
 They obtain the three unfolding curves shown below. 
  
 Which curves correspond to which forms of the protein?

Accepted Answer

Curve 1 = form B; Curve 2 = form A; Curve 3 form C

Answer

Curve 1 = form C; Curve 2 = form A; Curve 3 form B

Answer

Curve 1 = form A; Curve 2 = form C; Curve 3 form B

Answer

Curve 1 = form C; Curve 2 = form B; Curve 3 form A

Answer

Curve 1 = form A; Curve 2 = form B; Curve 3 form C

Answer

Curve 1 = form B; Curve 2 = form C; Curve 3 form A

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