Question 12: SOX10 and neural tube electroporation. [16 marks]. SOX10 is an ...
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Question 12: SOX10 and neural tube electroporation. [16 marks].
SOX10 is an important neural crest gene, normally expressed in neural crest cells in the dorsal part of the neural tube, and remains expressed as they migrate from the neural tube.
You suspect that expression of SOX10 might be sufficient to convert neural tube cells into migratory neural crest cells. To test this idea, you decide to ectopically express the neural crest gene SOX10 into the ventral part of the neural tube, together with a GFP reporter, using in ovo electroporation.a) What would you need to include in a plasmid/construct? What would you use as a control? (4 marks).b) Where would you place the DNA construct into the embryo? (2 marks). How would you electroporate the embryo? (2 marks).
To answer this question, upload a drawn image of where the DNA is placed and where the electrodes would be placed.
c) How does in ovo electroporation work to delivery DNA into cells? (3 marks). What particular advantage does the TOL2 vector have compared to so-called transient plasmids? (2 marks).
d) If your hypothesis is that SOX10 can convert neural tube cells into migratory neural crest cells, what result would provide evidence to support this hypothesis ? (3 marks).
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