You find that over-expression of the Shox2 gene in the neural tube of mouse produces a specific neural tube defect at E11.5. What method could you use to rapidly test if ectopic expression of the gene has the same or similar effect in the chicken embryo?

Question

You find that over-expression of the  Shox2  gene in the neural tube of mouse produces a specific neural tube defect at E11.5. What method could you use to  rapidly test  if ectopic expression of the gene has the same or similar effect in the chicken embryo?

Answer

Targeted deletion of the  Shox2  gene in chicken, using ES cells

Answer

Electroporation of Cas9 and  Shox2  guide RNAs into the chicken neural tube

Answer

In ovo  electroporation of  Shox2  into the neural tube of early chicken embryos

Answer

In situ hybridisation for  Shox2  mRNA expression in chicken embryos

Crowdly

You find that over-expression of the Shox2 gene in the neural tube of mouse pr...

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