A researcher is designing a zinc finger nuclease (ZFN) to
selectively disrupt a disease-causing gene in human cells. Precise targeting is
essential to minimise off-target double-strand breaks elsewhere in the genome.

Which feature of ZFNs provides the sequence specificity
required for accurate targeting of the desired DNA locus?

Question

A researcher is designing a zinc finger nuclease (ZFN) to
selectively disrupt a disease-causing gene in human cells. Precise targeting is
essential to minimise off-target double-strand breaks elsewhere in the genome.

Which feature of ZFNs provides the sequence specificity
required for accurate targeting of the desired DNA locus?

Accepted Answer

Modular
zinc finger domains, each recognising a specific three-nucleotide DNA sequence

Answer

Targeting
DNA sequences through an RNA guide molecule that directs ZFNs to their genomic
binding site

Answer

The
FokI nuclease domain, which binds selectively to target DNA sequences

Answer

Binding
to methylated CpG sites within the genome, allowing the nuclease to selectively
locate disease‑associated genes

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A researcher is designing a zinc finger nuclease (ZFN) to selectively disrupt a...

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