A researcher wants to study the development of endothelial cells, and decides to make a transgenic zebrafish to visualise endothelial cells using time-lapse imaging as they develop. What does the researcher need to make such a transgenic?

Question

A researcher wants to study the development of endothelial cells, and decides to make a transgenic zebrafish to visualise endothelial cells using time-lapse imaging as they develop.  What does the researcher need to make such a transgenic?

Accepted Answer

A line of fish expressing GFP under the influence of a promoter active in endothelial cells

Answer

A fluorescently-tagged morpholino driven by a promoter active in endothelial cells

Answer

Fish in which CRISPR/Cas9 has been used to delete an endothelial-expressed gene

Answer

An antibody that detects all endothelial cells, expressed off a GFP promoter

Crowdly

A researcher wants to study the development of endothelial cells, and decides to...

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