The DNA sequence encoding the Variable region of the immunoglobulin heavy chain was examined from a large number of B cells that had responded to the same antigen. Mutations were found in many of the V genes and some caused amino acid changes (replacements) and some did not (silent). What would you expect of the mutations causing amino acid changes?

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That they were concentrated in the complementarity determining regions (CDRs) of the V segments

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That they were concentrated in the framework regions (FWR) of the V gene

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That they were evenly distributed along the V segment

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That they reflected the distribution as first introduced by the enzyme AID

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The DNA sequence encoding the Variable region of the immunoglobulin heavy chain ...

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