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Drawing upon the resources and concepts covered in the Metabolism lab, and suppl...

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Drawing upon the resources and concepts covered in the Metabolism lab, and supplementing with some external research, complete the following:

1. Identify which specific tests in the Metabolism lab were used to:

     a. determine the presence of amylase, and

     b. determine the presence of maltose.

2. Consider the list of alternative techniques provided below. Select two alternative techniques from the list: one that would be appropriate for directly identifying amylase and one that would be appropriate for directly identifying maltose. You MUST select two different techniques, one for each molecule.

3. Compare and contrast your selected alternative techniques with the test(s) used in the Metabolism lab. Identify and describe at least one advantage and one disadvantage of the alternative technique when compared to the test(s) used in the Metabolism experiment (advantage = 50 words, disadvantage = 50 words).

4. Based on your comparison above, justify which of the two techniques (original or alternative) you would recommend for future use in this experiment (50 words).

Note 1: If more than two techniques are provided, only the first two will be considered and marked.

Note 2: If the same technique is used for both amylase and maltose, only the first instance will be marked. The second instance will receive zero marks.

Note 3: Answers using alternative techniques that are not on the list provided below will not be considered. These answers will receive zero marks.

Alternative Laboratory Techniques:

    • Gel electrophoresis
    • Barfoed test
    • Protein sequencing
    • Polymerase chain reaction (PCR)
    • Molisch's test
    • Fehling's test
    • High performance liquid chromatography (HPLC)
    • Mass spectrometry
    • Bial's test
    • Southern blot
    • Enzyme-linked immunosorbent assay (ELISA)

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