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A researcher analyses the binding between a protein and ligand by measuring the ...
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A researcher analyses the binding between a protein and ligand by measuring the intrinsic fluorescence of the protein in the presence of various different ligand concentrations (and using a protein concentration that is well below the Kd for ligand binding). The raw data are tabulated below.
[Ligand] (nM) | Raw Fluorescence (arbitrary units) |
0.0003 | 60 |
0.0010 | 60 |
0.0032 | 61 |
0.010 | 62 |
0.032 | 70 |
0.100 | 85 |
0.316 | 117 |
1.00 | 159 |
3.16 | 189 |
10.00 | 203 |
31.62 | 208 |
100 | 210 |
316 | 209 |
1000 | 209 |
3162 | 211 |
10000 | 209 |
Which of the following ligand concentrations gives a fractional saturation of the protein closest to 40%?
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