A plasmid vector for a particular gene of interest (shown in purple) was constructed and transfected into a suitable expression host. There was no expression of the gene of interest. Which one of the following statements is the best explanation for the observation.
Image failed to load: plasmid

Question

A plasmid vector for a particular gene of interest (shown in purple) was constructed and transfected into a suitable expression host. There was no expression of the gene of interest. Which one of the following statements is the best explanation for the observation.

Image failed to load: plasmid

Accepted Answer

The gene was inserted upstream of the promoter region

Answer

The gene was inserted downstream of the origin of replication

Answer

The gene was inserted outside of the multiple cloning site

Answer

None of these options explains the lack of expression

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A plasmid vector for a particular gene of interest (shown in purple) was constru...

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