The following is the PCR reaction that is programmed in the thermocycler to amplify the cheek cell DNA:

1. 95°C for 2 minutes
2. 95°C for 30 seconds
3. 58.1°C for 30 seconds
4. 72°C for 45 seconds
5. Repeat steps 2-4 thirty (30) times
6. 72°C for 3 minutes
7. Hold at 16°C indefinitely Which steps represent the denaturation, annealing and extension steps?

Question

The following is the PCR reaction that is programmed in the thermocycler to amplify the cheek cell DNA: 
 
 1.  95°C for 2 minutes 
 2.   95°C for 30 seconds 
 3.  58.1°C for 30 seconds      
 4.  72°C for 45 seconds 
 5.  Repeat steps 2-4 thirty (30) times 
 6.  72°C for 3 minutes 
 7.  Hold at 16°C indefinitely Which steps represent the denaturation, annealing and extension steps?

Answer

Step 2 = Denature; Step 4 = Anneal; Step 6 = Extend

Answer

Step 2 = Denature; Step 3 = Anneal; Step 4 = Extend

Answer

Step 3 = Denature; Step 4 = Anneal; Step 6 = Extend

Answer

Step 4 = Denature; Step 6 = Anneal; Step 7 = Extend

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The following is the PCR reaction that is programmed in the thermocycler to ampl...

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