An immunoassay for PAH detection in river sediments using polyclonal antibodies consistently reports higher contaminant levels than GC-MS analysis. What is the MOST LIKELY cause of this discrepancy?

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Answer

Cross-reactivity of the antibody with structurally similar PAHs

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Loss of sample antigen due to improper storage

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Interference from the sediment’s organic matter

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Signal molecule degradation during assay incubation

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An immunoassay for PAH detection in river sediments using polyclonal antibodies ...

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