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During Lab 4 you compared the amount of GFP under the control of four different ...
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During Lab 4 you compared the amount of GFP under the control of four different promoters, named Ara𝛃1, Ara𝛃2, Ara𝛃3 and Ara𝛃4. The following diagram (Figure 1) shows a sequence alignment between Ara𝛃2 and the wildtype promoter construct.
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Figure 1: Alignment between Ara𝛃2 and wildtype promoter constructs. The red boxes identify key sequences necessary for transcription (ie: AraC binding sites and the promoter sequence). Where the wildtype and Ara𝛃2 sequence is identical, a nucleotide base is shown in the consensus sequence. Where there is a difference between the wildtype and the Ara𝛃2 sequence a “+” sign is shown in the consensus sequence.The table below shows the relative fluorescence of the two strains obtained from Lab 4, the number of plus signs “+” gives an indication of the amount of fluorescence (ie: “+++” showed more fluorescence than “++”. A minus sign “-” indicates no fluorescence was observed).
| Induced (supplemented with arabinose) | Uninduced (not supplemented with arabinose) | |
|---|---|---|
| Wildtype | +++ | - |
| Ara𝛃2 (mutant) | ++ | - |