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Cette partie concerne les parties que vous n'avez pas compris dans les sections à lire. Écrivez en quelques mots les concepts qui vous ont semblé les plus difficiles à comprendre.

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On éclaire une plaque de d'aluminium, qui a une fonction d'extraction de 4,3 eV, avec de la lumière ultraviolette à 100 nm. Quelle sera la vitesse maximum des électrons éjectés par la plaque?

Donnez votre réponse en millions de m/s, arrondie à deux chiffres significatifs.

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Consider the following peptide sequence:

GSQANVMLTIHWEDCA

This peptide will be proteolytically cut by chymotrypsin, but not by trypsin.

What single amino acid substitution could you make that would prevent this peptide being cut by chymotrypsin but enable it to be cut by trypsin?

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Un électronvolt (eV) est égal à:

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Consider the following peptide sequence:

ACDEGHIKLMGNPQST

This peptide will be proteolytically cut by trypsin, but not by chymotrypsin.

What single amino acid substitution could you make that would prevent this peptide being cut by trypsin but enable it to be cut by chymotrypsin?

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Consider the following peptide sequence:

MVNNPQSTACDEGHIKL

This peptide will be proteolytically cut by trypsin, but not by chymotrypsin.

What single amino acid substitution could you make that would prevent this peptide being cut by trypsin but enable it to be cut by chymotrypsin?

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An eager young biochemist was digesting the same protein in three different tubes. Each tube had a separate protease:

  • trypsin
  • chymotrypsin, which cleaves at the carboxyl side of the three large and bulky aromatic amino acids
  • Glu-C, which cleaves C-terminally to Glu or Asp

After digestion, the samples were analysed by mass spectrometry but -- oh no! -- the biochemist forgot to label their tubes.

Luckily, they have you to help them.

Look at the sequences from each sample below to deduce (if possible) which enzyme had been used in which tube.

(Note: Given this was a large protein, the sequences provided are not an exhaustive list of all peptides observed, but should be enough for you to work with.)
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Un laser vert (longueur d'onde de 530 nm) émet 8 x 1017 photons par minute. 

Quelle est la puissance de ce laser?

Donnez votre réponse en mW, arrondie à deux chiffres significatifs.

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Vous augmentez l'intensité d'un faisceau lumineux sans changer sa fréquence. 

En termes de photons, celui signifie qu:

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Vous éclairez une plaque de métal avec de la lumière qui a une certaine fréquence f et vous remarquez qu'aucun électron n'est éjecté de la plaque. Pour commencez à éjecter des électrons, vous devriez:

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