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SC/BIOL 1000 M & N - Biology I (Winter 2025) (Labs)

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Why is it possible for you to study the eye colour gene by extracting cheek cells?

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What would happen if you set a micropipette outside its designated volume range? Select all answers that apply.

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Primers are pieces of single stranded DNA.

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The following is the PCR reaction that is programmed in the thermocycler to amplify the cheek cell DNA:

1.  95°C for 2 minutes

2.  95°C for 30 seconds

3.  58.1°C for 30 seconds     

4.  72°C for 45 seconds

5.  Repeat steps 2-4 thirty (30) times

6.  72°C for 3 minutes

7.  Hold at 16°C indefinitely

Which steps represent the denaturation, annealing and extension steps?

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Which of the following matches is not correct with regard to the PCR process?

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What is the purpose of the Phosphate Buffered Saline (PBS) in the DNA extraction protocol?

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After the DNA is released from the cell, it will be visible as a separate linear strands within the microtube. 
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Place the following statements regarding the steps of micropipetting in the correct order.

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What is the unit of volume that the micropipettes described in this lab dispense?

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What is the purpose of using a disposable tip on the micropipette?

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