Q2.

The researcher treated the

second batch of RNA with a MIXrobe kit that removes poly-adenylated RNA

transcripts using magnetic beads. Explain

why the MIXrobe kit does not remove bacterial mRNA. (1-2 sentences) (2 marks)

Q1. 

The researcher treated one batch

of the extracted RNA with the reagent Captcha which specifically binds to

7-methylguanosine. 

The forward primer for the polymerase chain reaction has been supplied

at a stock concentration of 5µM. The primer working concentration is

400nM. How many µl of the primer stock is needed to prepare 100µL of a 400nm solution?

Which of the following reagents can cause skin irritation, damage or burns? Select all that apply.

A 260/280 ratio of ____ is generally accepted as pure for DNA.

What personal protective equipment are you required to bring / wear to this practical? Select all that apply.

Which of the following reagents can cause eye irritation and/or eye damage? Select all that apply.

In sequential order, what are the three steps in PCR?

What item will be used to extract and purify genomic DNA?

Which of the following is not included in the polymerase chain reaction mastermix?