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MCB2011 - Molecular biology and the cell - S1 2025

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The ribbon model of the acyl-CoA dehydrogenase homotetramer shown above depicts:

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Which of the following hazard statements apply to the Assay Probe

(sodium dodecyl sulfate, 10% solution)? Select all that apply. HINT: You

need to search this chemical on the Chemwatch database.

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The promoter that controls transcription of the arabinose operon is called:

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When glucose is abundant the Catabolite Activator Protein (CAP):

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When setting up the colorimetric assay, in what order should the sample and reagents be added to each well?

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The assay probe has been provided at a stock concentration of 50µg/mL.

Calculate how many µL of probe must be diluted to prepare 1mL of a

1µg/mL solution.

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The phenomenon whereby Escherichia coli preferentially utilises glucose before any other carbon source is called:

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Which of the following components of the pGLO plasmid is a reporter gene?

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With respect to the arabinose operon, the AraC protein exerts ______________ transcriptional control.

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Q11. The DNA sequences of the manR

promoter in the non-mutated strain of

A. melonitis

and Mutants A and B

are shown below. The -10 sequence is underlined, the transcription start site

is indicated in bold and the start codon is highlighted in yellow.

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Use the

sequencing data above to explain why mannose is not metabolised by Mutant B? (2-3 sentences) (2 marks)

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