Compare the two DNA duplexes shown below.

Duplex 1                  Duplex 2

5′-CTAGCTCAAG-3′          5′-GATCGCATGTTC-3′

3′-GATCGAGTTC-5′          3′-CTAGCGTACAAG-5′

Which of the following statement(s) is/are correct?

An experiment was performed in which 250 mL of 0.7 M urea was used.

Calculate the number of millimoles (mmol) of urea contained in this sample.

Note: Be careful of your units!

An experiment requires you to create a stock buffer containing 0.2 M of NaCl in a total volume of 550 mL.

How many grams (g) of solid NaCl are required to prepare this stock solution?

Note: Your answer should be to two decimal places, e.g. 4.31

You took 3 mL of a stock solution of sodium phosphate (25 mM) and mixed it with 12 mL of water to make solution A.

You then took 1 mL of a solution A and mixed it with 1 mL of water to make solution B. 

You then took 1 mL of a solution B and mixed it with 4 mL of water to make solution C.

Calculate the concentration (in mM) of sodium phosphate in each of these solutions.

5 mL of a stock solution of 250 mM Tris buffer is diluted to a final volume of 25 mL.

What is the final concentration of the diluted Tris buffer (in units of mM)?

How many micromoles (μmol) of ATP are present in 6 mL of 5 mM ATP solution?

A sample contains 700 μg of NaOH.

How many milligrams (mg) is this?

A patient has been admitted to hospital and a blood test has shown his glucose level is 45 mg per 55 mL.

Calculate the molarity (in mM, to two decimal places) of the glucose in the patient's blood.

Shown below is a DNA duplex sequence, including both the coding (top) and non-coding (bottom) strands. 

Which one of the following pairs of oligonucleotide primers would be most suitable for amplifying (by PCR) the region of this duplex shaded in yellow?

[Note: You are not expected to estimate Tm values for these primers. If they have the correct sequence to anneal in the correct position, you should assume that they will.]