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BCH2011 - Structure and function of cellular biomolecules - S1 2025

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The following sequence of RNA forms a “hairpin” structure.

5′-CUGCAGCUCACGCACCAUGAGCUGAUGCUA-3′

What will be the effect on the stability of this hairpin structure if the bold, underlined nucleotide is mutated from A to C?

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Which of the following are nucleotide subunits typically found in RNA?

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The following sequence of RNA forms a “hairpin” structure. The duplex region contains all Watson-Crick base pairs without any gaps or mismatches.

5′-CGUAUACUAGUACUUAUGGUACUAGUUACU-3′

In the space below, type the sequence of either of the two regions that form a duplex with each other.

Be careful not to type any spaces or additional characters. For example, if you think the answer is 5'-AGCC-3', simply type AGCC.

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To answer this question, you must have plotted the data from the haemoglobin standard curve and answered the associated questions.

The patient who provided the blood sample is male. The levels of Hb are normal for this patient. True or false?

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To answer this question, you must have plotted the data from the haemoglobin standard curve.

How much Hb (in units of grams per litre) is present in the patient blood sample?

Note: enter your answer rounded to a whole number. E.g. if you think the answer is 15.4 g/L, then enter 15.

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To answer this question, you must have plotted the data from the haemoglobin standard curve.

How much Hb (in milligrams, mg) is present in Tube 6?

Note: enter your answer to one decimal place. E.g. if you think the answer is 15.42 mg, then enter 15.4.

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In this question you need to prepare a sodium phosphate standard curve across five tubes, with an amount of phosphate ranging from 0 μmol (in Tube 1) to 1.0 μmol (in Tube 5). The amount should increase evenly in 0.25 μmol increments.

You are provided with a solution of 1.25 mM sodium phosphate. Each tube must also contain 400 μL of reaction mix. The total volume in each tube should be 1.5 mL.

With this information, you should be able to complete the table below.

 TUBE 1TUBE 2TUBE 3TUBE 4TUBE 5
Sodium phosphateμmolμmolμmolμmolμmol
1.25 mM sodium phosphatemLmLmLmLmL
Reaction mixmLmL

mLmLmL
WatermLmL

mLmLmL

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You are preparing a tube containing 0.08 μmol of phosphate in a total volume of 6.0 mL.

What volume (μL) of 20 mM stock phosphate solution do you need to add into the tube?
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An experiment was performed in which 300 mL of 0.6 M urea was used.

Calculate the number of millimoles (mmol) of urea contained in this sample.

Note: Be careful of your units!

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How many micromoles (μmol) of ATP are present in 7 mL of 40 mM ATP solution?

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