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BCH2011 - Structure and function of cellular biomolecules - S1 2025

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An experiment requires you to create a stock buffer containing 0.15 M of NaCl in a total volume of 200 mL.

How many grams (g) of solid NaCl are required to prepare this stock solution?

Note: Your answer should be to two decimal places, e.g. 4.31

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6 mL of a stock solution of 300 mM citrate buffer is diluted to a final volume of 30 mL.

What is the final concentration of the diluted citrate buffer (in units of mM)?

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You took 2 mL of a stock solution of sodium phosphate (60 mM) and mixed it with 8 mL of water to make solution A.

You then took 1 mL of a solution A and mixed it with 3 mL of water to make solution B. 

You then took 2 mL of a solution B and mixed it with 2 mL of water to make solution C.

Calculate the concentration (in mM) of sodium phosphate in each of these solutions.

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A sample contains 70 μg of NaOH.

How many milligrams (mg) is this?

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An experiment requires the use of 5 mmol of monopotassium phosphate, which has a molecular mass of 136 Da.

What mass of monopotassium phosphate (in grams, to two decimal places) is needed for this experiment?

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A patient has been admitted to hospital and a blood test has shown his glucose level is 40 mg per 60 mL.

Calculate the molarity (in mM, to two decimal places) of the glucose in the patient's blood.

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Choose the best option to complete the following statement.

In the process of RNA synthesis, the problem of ensuring the correct sequence is made is solved by:

 

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Compare the two DNA duplexes shown below.

Duplex 1                  Duplex 2

5′-ACCGAGTCCG-3′          5′-ATAACTATGT-3′

3′-TGGCTCAGGC-5′          3′-TATTGATACA-5′

Which of the following statement(s) is/are correct?

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The following sequence of RNA forms a “hairpin” structure.

5′-UCGUGUGCUGCACCACAGCACAAUGCU-3′

What will be the effect on the stability of this hairpin structure if the bold, underlined nucleotide is mutated from G to U?

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The following sequence of RNA forms a “hairpin” structure. The duplex region contains all Watson-Crick base pairs without any gaps or mismatches.

5′-CGUAUACUAGACUGCUGGUCUAGUUACU-3′

In the space below, type the sequence of either of the two regions that form a duplex with each other.

Be careful not to type any spaces or additional characters. For example, if you think the answer is 5'-AGCC-3', simply type AGCC.

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