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BMS2052 - Microbes in health and disease - S2 2025

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The nuc gene is:

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Antibodies on the latex particles used in the BACTiStaph test bind to clumping factor and Protein A on the surface of Staphylococcus epidermidis.

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The BACTiStaph test is performed on:

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The catalase reagent (H2O2) is stored in the cold room in the PC2 laboratory. What does this imply about how the reagent should be handled?

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The catalase test is considered positive if bubbles are seen on the glass slide upon mixing bacterial cells with hydrogen peroxide. What do the bubbles indicate?

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What is the percentage of H2O2 used in the catalase test?

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The catalase test should not be performed using bacterial cells taken from a blood agar plate because blood cells:

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A student performed a Gram stain on a gram positive organism but forgot the Gram’s iodine step. What colour would the cells appear upon viewing with a light microscope?

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When preparing a heat fixed smear of bacterial cells taken from a plate culture, a loopful of ________ must be added to the glass slide to dilute the sample.

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