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DEV3011 - Experimental developmental biology and disease modelling - S1 2026
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Mouse embryos are often staged based on day of
embryogenesis, In mice, “E” = Embryonic days, and “
dpc” meansdays
postcoitum
. Using the images on page 37 of your DEV3011 Practical Manual, what
developmental stage (age) is your embryo? What features of your embryo have led
to this conclusion?
TASK 2. Staging zebrafish embryos
Question 2. Staging zebrafish embryos or larvae. [
You have a series of dishes containing zebrafish embryos or larvae at three different developmental stages. For each dish compare the embryos to the staging series images (see lab practical manual staging series ). Through this comparison and by counting the somite number, determine the stage of fishes in each dish.
[3 marks; 1 mark per dish]
Task 1: Anatomy of Zebrafish embryos
Observe the basic anatomy of the zebrafish embryos, using the dish containing the fish at 4dpf (dpf = days post-fertilisation).
Question 1. Zebrafish embryos. [7 marks.]Take a photograph of the embryo provided. Photographs can be uploaded onto Moodle. On your drawing or photograph clearly label and mark the following structures:
- Eye
- Pectoral fin
- Yolk
- Spinal chord
- Tail fin
- Otic vesicle (Hint: Look up "optic vesicle" online)
(7 marks; 1 mark for the image, 6 marks for the labelling).
worm will be available for viewing.
a) What is the fluorescent reporter gene being used in this worm ?b) Where in the worm is this reporter gene active?
c) What is the name of the gene it is reporting?