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DEV3011 - Experimental developmental biology and disease modelling - S1 2026

DEV3011 - Experimental developmental biology and disease modelling - S1 2026

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Inducible knockout mouse models permit temporal control over the induction of specific gene knockouts. Tamoxifen is commonly used to induce Cre-mediated recombination in mouse models. Which of the following is a component of this system?

A Cre-ER fusion protein

✅

Cre gene flanked by LoxP sites

❌

A Cre-Tamoxifen fusion protein

❌

A Tamoxifen-ER fusion protein

❌

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An Honours student plans to over-express Fgf10 specifically in the heart of mouse embryos. What is needed for this experiment?

An Fgf10 promoter that expresses in all cells.

A promoter that is active in the heart but not elsewhere.

100%

Fgf10 promoter driving LoxP expression in the heart.

A line of mice that expresses LoxP throughout the embryo

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You use Flp-FRT recombination in the eyes of Drosophila to investigate factors affecting growth and find that homozygous clones carrying a mutated (non-functional) gene “Tyrion” are smaller than wild-type clones. What would you conclude?

The gene “Tyrion” normally does not affect growth

The gene “Tyrion” normally inhibits growth

The gene “Tyrion” normally promotes growth

100%

The Flp-FRT recombination experiement failed

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In cells of the dorsal neural tube, BMP4 has just bound its Type II receptor. What is the first direct effect of this process?

Phosphorylation of Smad5

Activation of Type I receptor tyrosine kinase

Phosphorylation of serine and threonine resides

Dimerisation with type I receptor

100%

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A researcher is using a dominant modifier screen to study eye size in Drosophila

. The images below are of adult eyes with four different phenotypes in random order: wild type, sensitised, sensitised + enhancer mutant and sensitised + suppressor mutant.