You will set-up three separate PCR reactions in this laboratory class: experimental reaction, positive control reaction, negative control reaction. 

Which of the following reaction components are included in the negative control reaction? (select all that apply)

The PCR mastermix will be prepared in a sterile microtube that must be handled using aseptic technique. Why is this important?

Use the dilution equation (C1V1 = C2V2) to calculate the volume (µL) of 2mM dNTPs that is needed to prepare 100µL of PCR mastermix with a final dNTP concentration of 200µM.

DNA concentration and purity can be assessed by measuring the absorbance of ultraviolet (UV) light at three different wavelengths: 230nm, 260nm and 280nm. 

Which molecule absorbs UV light most strongly at 260nm?

A silica column-based method will be used to purify the patient's DNA. In the presence of ethanol and high salt, which molecule binds strongly to silica?

What is the primary function of the MFN2 gene product, mitofusin 2?

In this laboratory class you will use the polymerase chain reaction (PCR) to amplify:

Which of the following signs and symptoms of disease did your patient present with? (select all that apply)