Chemistry and Biochemistry
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PCR reaction - we
Determine the size of a PCR product amplified from the following genomic sequence by these primers:
Primer sequences:
F - 5’- TGTCCGTCGTGGATCTGAC -3’
R - 5’- CCTGCTTCACCACCTTCTTG -3’
Determine the size of a PCR product amplified from the following genomic sequence by these primers:
Primer sequences:
F - 5’- GCATTGAGAACCATGTGAGG -3’
R - 5’- TGCTTGGTGATGCTAATGCT -3’
3% agarose gel is used for the electrophoresis of DNA fragments. Agarose is:
Which of the following restriction fragments will move the fastest during electrophoresis?
In biochemistry, concentrated stock solutions (n× concentrated) are often used, which are diluted to the working concentration (1×) just before use.
What volume of buffer labeled
TAE 20× must we add to the resulting volume of 150 mL of solution?
Match the restriction enzyme responsible for the
cleavage with the corresponding DNA sequence. The figure shows the restriction
sites of the restriction endonucleases used:
Reverse transcriptase is an enzyme, which catalyses:
Which of the following numbers is a 'palindrome'?
Which of the 'blotting' techniques was the first and was named after the one who introduced it to molecular biology?
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