Chemistry and Biochemistry
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Restriction endonucleases are enzymes of:
Determine the size of a PCR product amplified from the following genomic sequence by these primers:
Primer sequences:
F - 5’- GGCAGAGAAGAGAGTGTTTGC -3’
R - 5’- TCTTCTTTCTCCCAGCCCTA -3’
PCR reaction - we do not need:
Determine the size of a PCR product amplified from the following genomic sequence by these primers:
Primer sequences:
F - 5’- GCATTGAGAACCATGTGAGG -3’
R - 5’- TGCTTGGTGATGCTAATGCT -3’
PCR reaction - we need
Determine the size of a PCR product amplified from the following genomic sequence by these primers:
Primer sequences:
F - 5’- TGTCCGTCGTGGATCTGAC -3’
R - 5’- CCTGCTTCACCACCTTCTTG -3’
PCR reaction - we do not need:
Choose which of the listed pairs of primers is already unusable at first glance.
Determine the size of a PCR product amplified from the following genomic sequence by these primers:
Primer sequences:
F - 5’- GGCGCTACCTGTATCAATGG -3’
R - 5’- TCAGCCAACTCGTCACAGTC -3’
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