Enzyme-linked detection system

Gel electrophoresis

PCR amplification

Radioactive labeling

To denature proteins and give them a uniform negative charge

To help proteins bind to the membrane

To block nonspecific binding on the membrane

To detect specific proteins via a color change

Polypropylene membrane

Nitrocellulose membrane

Silicone membrane

Agarose membrane

To detect the specific protein of interest

To bind to the primary antibody and amplify the signal

To block nonspecific binding sites on the membrane

To separate proteins based on their size

Blocking the membrane

Separation of proteins by SDS-PAGE

Antibody incubation

Protein transfer to a membrane

To sequence proteins in a sample

To visualize RNA expression levels

To separate DNA fragments based on size

To identify and quantify specific proteins in a sample