Once the DNA at the replication fork is unwound by helicases, what prevents the two strands from coming back together to re-form a double helix?

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Answer

The helicase modifies the DNA in such a way as to eliminate the affinity between the two strands.

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Single-strand binding proteins bind the unwound DNA and prevent the double helix from re-forming.

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The helicase pushes the two strands so far apart that they have no chance of finding each other.

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One of the strands is rapidly degraded, preventing the double helix from re-forming.

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DNA polymerase follows the helicase so closely that there is no chance for the strands to come back together.

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