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Proteins to be imported into the nucleus:
first require phosphorylation in the cytosol at the nuclear localization signal
contain a nuclear localization signal when they are larger than ~50kDa or for their efficient import when they are less than ~50 kDa
need to be kept in an unfolded conformation by cytosolic chaperones
are first imported into the ER before binding importin and undergoing vesicle-mediated transport to the nuclear pore
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