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The spliceosome cuts the pre-mRNA two times (once at the 5’ splice site & once at the 3’ splice site) to remove each intron to form the mature mRNA.
In the image below, we can see that the pre-mRNA can be used to create different mature mRNAs (names V1, V2, V3) through alternate splicing. How many times did the spliceosome cleave the pre-mRNA to create the mature mRNA V1 shown below?