Sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) is a technique commonly used in protein biochemistry. This technique allows analysis of proteins by separating them on a polyacrylamide gel.
Why are proteins to be run on sodium dodecyl sulphate (SDS) electrophoresis gels denatured and reduced?

Question

Sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) is a technique commonly used in protein biochemistry.  This technique allows analysis of proteins by separating them on a polyacrylamide gel. 
 Why are proteins to be run on sodium dodecyl sulphate (SDS) electrophoresis gels denatured and reduced?

Answer

To ensure that large proteins move more quickly through the gel

Answer

To remove primary and secondary structures, allowing proteins to move through the gel

Answer

To ensure that protein separation is based solely on charge

Answer

To separate the proteins into their subunits by removing tertiary and quaternary structures

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Sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) is a techn...

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