Which of the following statements accurately describe Gene Set Enrichment Analysis (GSEA) in RNA sequencing studies? Select all that apply

Question

Accepted Answer

GSEA evaluates the collective behavior of a group of genes, rather than analyzing genes individually

Accepted Answer

GSEA can reveal biological processes and pathways that may not be evident from individual gene-level analysis.

Answer

GSEA identifies whether predefined gene sets (e.g., pathways) are significantly enriched in the ranked list of genes from an experiment

Answer

GSEA cannot be applied to RNASeq studies

Answer

GSEA requires setting a hard threshold for defining differentially expressed genes before performing the analysis

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