The research team is investigating which specific biomolecule in the bacteria ...
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The research team is investigating which specific biomolecule in the bacteria is chiefly responsible for causing the disease. They aim to identify it using a combination of radiolabelling, chemical assays, and cell fractionation.
The following experimental results are obtained:
Radiolabelling Experiments:
•The biomolecule
is labelled
when
bacteria are grown in a medium containing ¹⁵N (nitrogen-15).
•No
labelling is
observed when bacteria are grown with ³²P (phosphorus-32).
Chemical Assays:
•It shows
high
catalytic activity
when tested for a specific biochemical reaction.
Cell Fractionation:
•The biomolecule
is primarily found in the cytoplasmic fraction
after ultracentrifugation.
(i) Based on these observations, one of the research member initially thinks
it’s a ribozyme. Can you give one reason for this hunch, and one way to falsify
this? ( 1 mark)
(ii) Can you suggest which molecule it likely is, and suggest one more test/experiment that could help confirm the nature of the molecule? (1 mark)Want instant access to all verified answers on dle.plaksha.edu.in?
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