For the following restriction enzymes (REs), the recognition sequences and the cut sites are shown on the template strand (all reading left to right as 5' to 3'), indicate which REs create sticky ends and which ones create blunt ends.

Which one of the following statements best explains why DNA replication is termed semi-conservative?

The following mature (spliced) mRNA sequence from a gene is provided to you in-frame.

5' - A G C C U A U C C A - 3'

Using the codon table below, which one of the following options gives the correct sequence of amino acids when you translate this segment of mRNA?

A plasmid vector for a particular gene of interest (shown in purple) was constructed and transfected into a suitable expression host.  There was no expression of the gene of interest.  Which one of the following statements is the best explanation for the observation.

After using dideoxy nucleotides (ddNTPs) in the chain termination (Sanger) method for sequencing a DNA fragment, you observe the following:

Based on the sequencing gel shown above, where the arrow indicates the direction of electrophoresis, which one of the following is the sequence of the original strand of DNA (the strand that was sequenced, that is, was the template for the sequencing reaction)? 

In 15 words or less, briefly explain the purpose of the "LB / amp; -pGlo" plate.

In 15 words or less, briefly explain the purpose of the "LB / -pGlo" plate.

Predict what would happen if you took one of the white colonies from the LB/amp plate, streaked it onto an LB/amp/ara plate, and then after incubation, examined the plate under UV light.