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MCB2011 - Molecular biology and the cell - S1 2025
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Q5. RT-PCR was performed on the enriched plant RNA using region-specific primers for the gene. The RT-PCR products were analysed by gel electrophoresis and the results are shown in the image below.
Which band (1, 2 or 3) corresponds with mRNA
transcript C?
Q11. The DNA sequences of the manR promoter in the non-mutated strain of and Mutants A and B are shown below. The -10 sequence is underlined, the transcription start site is indicated in bold and the start codon is highlighted in yellow.
Use the sequencing data above to explain why mannose is not metabolised by Mutant B? (2-3 sentences) (2 marks)
Q10. What kind of protein is ManR likely to be?
Based on the data in the above graphs, in which
mutant is carbon catabolite repression abolished? Briefly justify your response. (2-3 sentences) (2 marks)
Q8. Which of the following molecules are involved in the global regulation of catabolic operons? Select all that apply.
Q7. Which of the following statements apply to the regulation of catabolic operons? Select all that apply.
Q5. RT-PCR was performed on the enriched plant RNA using region-specific primers for the gene. The RT-PCR products were analysed by gel electrophoresis and the results are shown in the image below.
Which band (1, 2 or 3) corresponds with mRNA
transcript C?
Q6.The first 10 nucleotides of the first exon of the ProfX gene are 5’ ATG GGT TCC T 3’. What is the sequence of the first 10 nucleotides of transcribed mRNA in the pre-mRNA transcript?
Q3. What type of splicing most likely produced mRNA transcript A?
Q4. What type of splicing most likely produced mRNA transcript B?