Q4. As we don’t yet know the chromosomal location of the HB2011 gene, which method from the options in this diagram would be most appropriate to ascertain the gene’s location?

Q12. Your supervisor is considering altering the HB2011 gene in human embryos. What is the main ethical issue associated with germline gene editing?

Q11. Describe what happens to the CRISPR/Cas9 double stranded break in the mi2011 gene in the absence of a repair template. (1 sentence, 2 marks)

Q10. When designing your CRISPR experiments to genetically edit mi2011, what should you take into consideration regarding the PAM sequence?

Q8. Your next aim within the project is to design a knockout mouse model of the HB2011 gene. Out of the options discussed in the module, explain in brief detail which would be best to use and why? (2 marks)

Q7. On average, how often would enzyme 3 cut DNA?

Q6. Explain why you chose this enzyme in Q5 to cut the DNA. (2 marks)

Q5. Which of the restriction enzymes would you use to cut the DNA sequence upstream of the gene to insert into your plasmid?

Q4. As we don’t yet know the chromosomal location of the HB2011 gene, which method from the options in this diagram would be most appropriate to ascertain the gene’s location?

a) Discuss the ethical principle that was upheld during the research (1 mark)

b) Name and discuss an ethical principle that was not upheld in this research (1 mark)