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BIO1011 - Blueprints for Life - Clayton S1 2025

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You apply a drug to a cell that binds to the centromere of chromosomes and blocks the attachment of spindle fibres. At which stage of mitosis will the effect of this drug first be seen?

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If the template strand of DNA is of the sequence 5’-CCTGAA-3’, what sequence will DNA polymerase synthesise?

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You are observing the cells of an organism that is diploid undergoing meiosis. Which stage does the image above represent?

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In the above image, which of the numbered circles indicate the 3’ end of the nucleotide?

 

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Which of the following options are the main functions of the G2/M cyclin/ CDK complex? 

  1. To ensure that the components required for DNA synthesis are available

  2. To ensure the cell is ready to enter interphase

  3. To confirm that newly synthesised DNA fragments are not damaged

  4. To ensure the enzymes responsible for spindle fibre formation are produced

 

 

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During which of the following stages of the mitotic cell cycle will the cell contain the same amount of DNA that a non-dividing cell usually contains?

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 Scatterplot Graph 

Using the average absorbance values from the 8 replicates for each treatment, construct an XY scatterplot graph (using Excel) with trendlines using linear regression (R2) for each treatment. Upload this graph here.

NOTE: Students need to be proficient at constructing graphs (for future assessments) and this provides an opportunity to practice using the software while you can get assistance from your peers. TA’s will NOT assist with graph construction during the lab session.

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 Prelab Activity 

Upload your prelab activity here. Remember that your hypothesis should make reference to all treatments and include a justification for each.

Once you have collated all the results from your lab session, indicate in your prelab activity table whether the results supported each component of your hypothesis or not.

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 Lab 3 Results 

Save your lab results! Upload your Lab 3 results here for future reference.

Choose one of the following:

  • Best way: Transcribe the whiteboard data into an Excel file or the tables in your results sheet document. This makes it easier to use for your subsequent assignments.
  • Quick way: Take a photo of the whiteboard containing all results.

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As with all spectrophotometric measurements, it is essential to use a blank.

From the list of reagents provided below, select all the components required to make up the blank.

Note: Multiple options may be correct. Marks are deducted for each incorrect selection.

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