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BCH2011 - Structure and function of cellular biomolecules - S1 2025

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The following sequence of RNA forms a “hairpin” structure. The duplex region contains all Watson-Crick base pairs without any gaps or mismatches.

5'-AAACCGGAUAAUUGGGAUUAUCCGGUUAGG-3'

In the space below type the sequence of either of the two regions that form a duplex with each other. Be careful not to type any spaces or additional characters. (Do not enter in 5' or 3'. For example, if a potential answer was 5'-AAUUGG-3' then you would simply type: AAUUGG.)

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The following sequence of RNA forms a “hairpin” structure. The duplex region contains all Watson-Crick base pairs without any gaps or mismatches.

5'-AAAGGAUUAGGGCAACGGCCUGCCCUAAUG-3'

In the space below type the sequence of either of the two regions that form a duplex with each other. Be careful not to type any spaces or additional characters. (Do not enter in 5' or 3'. For example, if a potential answer was 5'-AAUUGG-3' then you would simply type: AAUUGG.)

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Shown below is data from an experiment assessing the activity of an enzyme following Michaelis-Menten kinetics.

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What would the Vmax (in units of micromolar/min) of this reaction be if one third the amount of enzyme had been used for the experiment?

(Note: do not enter any units. You just need to enter in the numerical value, e.g. 15. Obviously the answer isn't 15 or I wouldn't have written that. I guess I could be weird and try to throw you by writing that and having the answer as actually that. But no, I wouldn't do that to you. Plus, you know the answer isn't 15. You've already worked it out and stopped reading this nonsense I'm still typing...)

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One strand of a DNA duplex has the sequence 5'-ACCGACTTGA-3'

Type out the other strand, written in the 5' to 3' direction.

(Do not include 5', 3' or any character other than nucleotides. E.g. if you think the answer is 5'-AAAA-3', type: AAAA)

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The following data were obtained in a study of an enzyme and substrate A, known to follow Michaelis-Menten kinetics:

Image failed to load

Estimate the Km (in units of micromolar) for the enzyme substrate reaction.

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The following data were obtained in a study of an enzyme and substrate A, known to follow Michaelis-Menten kinetics:

Image failed to load

Estimate Vmax in units of micromolar/min. You do not need to enter unit, only a value.

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Which one of the following anticodons would bring in the amino acid proline?

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Note: you'll have already seen a question with the same image below. The question order shouldn't be randomised. There are a few questions with this same image.

The image below shows the titration curve for a dipeptide (think what that means).

The pI for this dipeptide is 5.0

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What would the average charge of this dipeptide be at a pH of 8?

(You should be able to work this out even without knowing the precise identity of the amino acids.)

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Which one of the following non-coding strands of DNA can be transcribed into mRNA to encode the polypeptide sequence MetLeuGlyAsp?

(Note: Assume standard initiation of translation, but do not assume there is a stop codon after the codon for Asp. Assume that the sequence continues on...)

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Amino acids are attached to tRNAs via an:

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