The sequence below represents the coding DNA strand of the start of a gene, including part of the 5' untranslated region.

(5')...CCGGAAGGAGGTAACCGCAATGACCGGGCCTTTGAACC...(3')

In studying disease variants, a nucleotide mutation of T→A was detected in the second base of the fifth codon. What effect would this mutation have? 

(When considering your answer, assume standard initiation of translation is numbered as codon 1.)

The image below shows the three main sites -- E, P and A -- of the ribosome involved in the process of translation.

A new inhibitor of translation has been found that occupies the "A" site.

What specific effect would this have on translation?

Consider the following coding strand DNA sequence, written 5'-to-3':

TCTTGGGAAGAGACC

If this sequence were to be directly translated from the first base, what is the amino acid sequence (in single letter code) that would arise?

(Your answer should only include single-letter amino acids. Do not include spaces or other non-amino acid characters.)

In the standard genetic code, 61 of the 64 different codons code for the 20 common amino acids. What function do the remaining three codons serve?

Consider the following tripeptide:

Glu-Tyr-Ile

In the box below, enter a minimal DNA sequence (coding strand), written 5'-to-3', that could give rise to this peptide. Do not include an initiating codon at the start. Please just enter in the codons for the given peptide above.

(You should only include nucleotides in your answer, e.g. AAAA... Do not include 5' or 3', spaces, or any other non-nucleotide characters.)

The image below shows the three main sites -- E, P and A -- of the ribosome involved in the process of translation.

A new inhibitor of translation has been found that occupies the "E" site.

What specific effect would this have on translation?

During the process of translation, which ONE of the following statements is true?

Consider the following tripeptide:

Val-Gln-Asn

In the box below, enter a minimal DNA sequence (coding strand), written 5'-to-3', that could give rise to this peptide. Do not include an initiating codon at the start. Please just enter in the codons for the given peptide above.

(You should only include nucleotides in your answer, e.g. AAAA... Do not include 5' or 3', spaces, or any other non-nucleotide characters.)

Using your knowledge of the genetic code and the image below, which ONE of the following amino acid replacements can be caused by a single nucleotide change?

Consider the following mRNA (5'-to-3') codon:

CAG

Assuming standard base-pairing (i.e. no 'wobble'), enter the corresponding tRNA anticodon written in the 5'-to-3' orientation.

(Your answer should only include nucleotides, e.g. UUU. Do not enter 5' or 3'.)